Cytotoxic Effects of Artemisia annua Methanol Extract on Cancer Cell Lines in Vitro

نویسندگان

  • A Ahi Researcher, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • A Emami Associate Professor of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
  • M Mahmoudi Professor of Immunology, Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
  • SH Zamani Taghizadeh Rabe Master of Science in Immunology, Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده مقاله:

Background & Aims: In most cases, drugs used for the treatment of cancer are not effective or have unpleasant side-effects. This has forced scientists to find more effective drugs with less toxicity. Artemisia is an important medicinal plant in the world and anti-tumoral activity of some Artemisia species has been reported. This study was designed for evaluation of anti-tumoral effect of methanol extract isolated from Artemisia annua on different cancer cell lines. Methods: Methanol extract of Artemisia annua was prepared. Cultivated gastric (AGS), cervix (Hela), colon (HT-29) and breast (MCF-7) cancer cell lines and normal fibroblast cells (L929) were incubated with different concentrations of methanol extract for 72 hours and cell growth inhibition was determined using MTT assay. Results: Results of MTT assay showed strong and dose-dependent inhibition of cancer cell growth by methanol extract of Artemisia annua. This extract caused a significant decrease in proliferation of tested cancer cell lines and had less toxicity on normal cells. Conclusion: Since the results suggest anti- tumoral activity of Artemisia annua, isolation of effective compound/s of this extract and evaluation of its/their effects on tumor-bearing animal models are suggested.

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عنوان ژورنال

دوره 17  شماره 3

صفحات  215- 225

تاریخ انتشار 2010-07-01

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